pro togelTo pro-vide a sharp interface, overlay the gel with wa - ter saturated n-butanol during polymerization. Flush butanol away with water just before cast - ing the stacking gel (below). 3.The use of National Diagnostics’ ProtoGel Stacking Buffer will ensure the purity and performance of your Laemmli gels. When diluted as directed ProtoGel Stacking Buffer forms a gel of 0.125